Anti-F-actin antibody [4E3.adl] ab130935 from Abcam

Product Specs
ItemAnti-F-actin antibody [4E3.adl]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab130935
Quantity10 UG, 100 UG, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Conjugate/TagUnconjugated
ClonalityMonoclonal
IsotypeIgM
Clone4E3.adl
HostMouse, Nude mouse
Antibody TypePrimary
Gene NameACTA1
ReactivityHuman, Mouse
TargetF-actin

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(1) Silencing Glypican-1 enhances the antitumor effects of Pictilisib via downregulating PI3K/Akt/ERK signaling in chemo-resistant esophageal adenocarcinomaMolecular & Cellular OncologyDecember 31, 2023Akshay Pratap, Andrea Qualman, Hedlund Garrett, Lindsey Westbrook, Erlinda The, Sanchayita Mitra, Mila Cordero, Kenneth Meza Monge, Juan- Pablo Idrovo, Argudit Chauhan, et al.Signaling,1:1000 ERK1/2,Cell Signaling,Cell Signaling,1:1000 AKT,Cell Signaling,4691,1:1000 F-Actin,Abcam,ab130935,1:1000 SNAIL 1,Proteintec,13099–1-AP,1:1000 Vimentin,Proteintec,10366-AP,1:1000 Beta Actin,Proteintec,66009–1-Ig,"1:10,000" Secondary

(2) Inhibition of TGF-β2–Induced Trabecular Meshwork Fibrosis by PirfenidoneTranslational Vision Science & TechnologyNovember 17, 2023Xiaofeng Zhu, Bei Zeng, Caiqing Wu, Zidong Chen, Minbin Yu, Yangfan YangNo." Anti-myocilin,1:2000,—,"Proteintech, 60357" Anti-α-SMA,1:10000,1:500,"Abcam, ab124964" Anti-F-actin,1:500,1:500,"Abcam, ab130935" Anti-collagen IV,1:1000,1:500,"Abcam, ab6586" Anti-fibronectin,1:1000,1:500,"Abcam, ab2413" Primary

(3) ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistanceDiscover OncologyOctober 24, 2023Chao Xu, Yongjie Xie, Peng Xie, Jianming Li, Zhongsheng Tong, Yanfang Yangincubated with primary antibodies against ZDHHC9 (Proteintech, 24046-1-AP, 1:100) and F-actin (Abcam, ab130935, 1:200) overnight at 4 ℃. The following day, the cells were incubated with fluorescently labelled secondary

(4) hucMSCs treatment prevents pulmonary fibrosis by reducing circANKRD42-YAP1-mediated mechanical stiffnessAging (Albany NY)June 16, 2023Haitong Zhang, Qi Zhu, Yunxia Ji, Meirong Wang, Qian Zhang, Weili Liu, Ruiqiong Li, Jinjin Zhang, Pan Xu, Xiaodong Song, et al.(Cell Signaling Technology, 14074S), anti-phospho-YAP1 (Cell Signaling Technology, 53749S), anti-F-actin (Abcam, ab130935), anti-GAPDH (Affinity, AF7021). 1×Tris buffered saline Tween was used to wash the membranes for three

(5) The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissuesGraefe's Archive for Clinical and Experimental OphthalmologyMay 1, 2023Rossella Colabelli Gisoldi, Gemma Lodato, Bijorn Omar Balzamino, Graziana Esposito, Alessandra Micera, Augusto Pocobelli1:1000; sc-374429; Santa Cruz Biotech, Santa Cruz, CA), F-actin (anti-F-actin antibody, [4E3.adl], ab130935; predicted/observed band size at 42/37 kDa; 1:1000; Abcam, Cambridge, UK), and VEGF (anti-VEGF 165A

(6) The Slingshot phosphatase 2 is required for acrosome biogenesis during spermatogenesis in miceeLifeMarch 21, 2023Xu K, Su X, Fang K, Lv Y, Huang T, Li M, Wang Z, Yin Y, Muhammad T, Liu S, et al.dilution, sc-365414, Santa Cruz Biotechnology), mouse anti-F-ACTIN monoclonal antibody (1:500 dilution, ab130935, Abcam), rabbit anti-BAX antibody (1:1000 dilution, 2772, Cell Signaling Technology), rabbit anti-BAD

(7) Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodiaIran J Basic Med SciJanuary 1, 2023Huisi Lin, Yujuan Fan, Zhifu Zhi, Lihong Pang, Dan Sun(ab33186), rabbit monoclonal anti-cortactin antibody (ab81208), and primary antibody-like anti-F-actin (ab130935) were obtained from Abcam (Cambridge, MA, USA), while the primary antibody anti-Rac1 (bs-4186R) was

(8) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasisCardiovascular DiabetologyDecember 1, 2022Rongjun Zou, Wanting Shi, Junxiong Qiu, Na Zhou, Na Du, Hao Zhou, Xinxin Chen, Li MaDrp1 (#ab184247), Fis1 (#ab156865), GAPDH (#ab8245), Tom20 (#ab186735), DNA-PKcs (#ab32566), F-actin (#ab130935) and Met (#ab51067). The following antibodies were obtained from Cell Signaling Technology: p-Drp1 (#4494),

(9) Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axisCell Communication and SignalingDecember 1, 2022Siyuan Yin, Ru Song, Jiaxu Ma, Chunyan Liu, Zhenjie Wu, Guoqi Cao, Jian Liu, Guang Zhang, Huayu Zhang, Rui Sun, et al.anti-YAP (#14074), and anti-CREB (#9197), all diluted 1:200 and purchased from CST, and rat anti-Actin (#ab130935) was diluted 1:200 and purchased from Abcam. CUT & RUN assay Protein-DNA interactions were analysed

(10) Transfer of microRNA-22-3p by M2 macrophage-derived extracellular vesicles facilitates the development of ankylosing spondylitis through the PER2-mediated Wnt/β-catenin axisCell Death DiscoveryDecember 1, 2022Chong Liu, Tuo Liang, Zide Zhang, Jiarui Chen, Jang Xue, Xinli Zhan, Liang Ren4′,6-diamidino-2-phenylindole (DAPI) (blue), after which the cytoskeleton was stained green using F-actin (ab130935, Abcam). Then, the cells were incubated at 37 °C for 12 h. The uptake of M2-EVs by cells was visualized

(11) Endothelial Barrier Disruption by Lipid Emulsions Containing a High Amount of N3 Fatty Acids (Omegaven) but Not N6 Fatty Acids (Intralipid)CellsJuly 14, 2022Emilie Gueguen, Yasser Morsy, Michael Scharl, Stefanie D. Krämer, Michael Zaugg, Martin Hersberger, Gerhard Rogler, Marcin Wawrzyniakthe ProLong™ Gold Antifade Mountant with DAPI (P36935-Thermo Fisher Scientific). Anti-human F-actin (ab130935) and anti-human VE-Cadherin (ab33168) (Abcam, Cambridge, United Kingdom) antibodies were used in the

(12) Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoidsChinese Medical JournalFebruary 5, 2022Lijie Du, Lei Zhang, Junhong Zhao, Zixiu Chen, Xiang Liu, Manxiu Cao, Lei You, Yonghong Zhang, Xiaobing Fu, Haihong Liand 3C]. The corresponding antibodies are as follows: Ki67 (Invitrogen SP6, 1:1000), F-actin (Abcam ab130935, 1:500), LC3B (CST 3868T, 1:1000), PARP (CST 9542, 1:1000), cleaved caspase-3 (CST 9664 s, 1:1000),

(13) Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneuronsNeural Regeneration ResearchJanuary 12, 2022Qian-Ru Ding, Qian-Ru Cong, Fan-Hui Cong, Fan-Hui Yu, Yu-Hua Ji, Hai-Yan Shi, Hai-Yan Dinganti-p-TrkB (MilliporeSigma, Cat# ABN1381, RRID: AB_2721199, 1:500), mouse anti-F-actin antibody (Abcam, Cat# ab130935, 1:400), mouse anti-β-Actin antibody (Abcam, Cat# ab8224, RRID: AB_449644, 1:400), and rabbit anti-ChAT

(14) SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathwaysTheranosticsJanuary 1, 2022Ma L, Zou R, Shi W, Zhou N, Chen S, Zhou H, Chen X, Wu Y.Millipore), and incubated overnight at 4 °C with corresponding primary antibodies: F-actin (1:1000, Abcam, #ab130935), G-actin (1:1000, Abcam, #ab200046), p-CaMKII (1:1000, Abcam, #ab124880), cofilin (1:1000, Abcam, #ab54532),

(15) Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin PathwayFrontiers in medicineJanuary 1, 2022Feng L, Li H, Du Y, Zhang T, Zhu Y, Li Z, Zhao L, Wang X, Wang G, Zhou L, et al.with different primary antibodies: anti-MERTK (Cell Signaling Technology, 4319S), anti-F-actin (Abcam, ab130935), anti-CCT5 (Proteintech, 11603-1-AP), anti-cofilin (Abcam, ab54532), anti-phosphorylated cofilin (p-cofilin,

(16) Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1Frontiers in Cell and Developmental BiologyMarch 12, 2021Li Zhang et al.binding, the membrane was incubated in different primary antibodies: CFL1 (CST #5175), F–actin (Abcam#ab130935), G–actin (Abcam#ab123034), GAPDH (Abcam#ab128915). All antibodies were used at a 1:1,000 dilution,

(17) Mechanical stretch promotes hypertrophic scar formation through mechanically activated cation channel Piezo1Cell Death & DiseaseMarch 1, 2021Jiahao He, Bin Fang, Shengzhou Shan, Yun Xie, Chuandong Wang, Yifan Zhang, Xiaoling Zhang, Qingfeng Lisamples: anti-Piezo1 (Abcam, ab128245, 1:100), anti-Ki67 (CST, #9449, 1:400), anti-F-actin (Abcam, ab130935, 1:500), anti-α-SMA (CST, #48938, 1:200), Alexa Fluor 594 goat anti-rabbit secondary antibody (Jackson

(18) Nerve growth factor regulates liver cancer cell polarity and motilityMolecular Medicine ReportsFebruary 19, 2021Haihua Lin, Huanjun Huang, Yuhong Yu, Weibin Chen, Shaomin Zhang, Yuanzhi ZhangInc.), vimentin (1:1,000; cat. no. 3932; Cell Signaling Technology, Inc.), F‑actin (1:1,000; cat. no. ab130935; Abcam) and β‑actin (1:1,000; cat. no. sc69879; Santa Cruz Biotechnology Inc.) were used for different

(19) ICAM-1 Activates Platelets and Promotes Endothelial Permeability through VE-Cadherin after Insufficient Radiofrequency AblationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2021Kong J, Yao C, Dong S, Wu S, Xu Y, Li K, Ji L, Shen Q, Zhang Q, Zhan R, et al.antibodies including anti‐VE‐cadherin (1:200; ab33168, Abcam, London, UK) and anti‐F‐actin (1:200; ab130935, Abcam, London, UK) overnight at 4 °C. Plates were washed and were then incubated with anti‐mouse or

(20) Radiation-induced abscopal reproductive effect is driven by TNF-α/p38 MAPK/Rac1 axis in Sertoli cellsTheranosticsJanuary 1, 2021Hu S, Zhu L, Song Y, Zhao X, Chen Q, Pan Y, Zhang J, Bai Y, Zhang H, Shao C.(ab51129, Abcam), TNF-α (ab183218, Abcam), ZO-1 (ab216880, Abcam), Occludin (33-1500, Invitrogen), F-actin (ab130935, Abcam), p38 (ab170099, Abcam), p-p38 (ab195049, Abcam), β-actin (AF0003, Beyotime), α-tubulin (AF0001,

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Anti-F-actin antibody [4E3.adl] from Abcam

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Anti-F-actin antibody [4E3.adl] from Abcam

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